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mdh malate dehydrogenase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947854, updated on 23-Nov-2023

Summary

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Gene symbol
mdh
Gene description
malate dehydrogenase
Primary source
ASAP:ABE-0010613
Locus tag
b3236
See related
ECOCYC:EG10576
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3225
Summary
Mdh is elevated 4X during growth at 4C (enabled by Oleispira antarctica Cpn10/60 transgene). [More information is available at EcoGene: EG10576]. Malate dehydrogenase (Mdh) catalyzes the reversible oxidation of malate to generate oxaloacetate. [More information is available at EcoCyc: EG10576].
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Genomic context

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Sequence:
NC_000913.3 (3383330..3384268, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene periplasmic serine endoprotease Neighboring gene serine endoprotease Neighboring gene DNA-binding transcriptional dual regulator ArgR Neighboring gene DUF1471 domain-containing stress-induced protein YhcN

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Studying the Lysine Acetylation of Malate Dehydrogenase. Venkat S, et al. J Mol Biol, 2017 May 5. PMID 28366830, Free PMC Article
  2. Structure of Escherichia coli malate dehydrogenase at 1.45 A resolution. Zaitseva J, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009 Sep 1. PMID 19724119, Free PMC Article
  3. Identification of functional paralog shift mutations: conversion of Escherichia coli malate dehydrogenase to a lactate dehydrogenase. Yin Y, et al. Proc Natl Acad Sci U S A, 2007 Oct 30. PMID 17947381, Free PMC Article
  4. Escherichia coli malate dehydrogenase, a novel solubility enhancer for heterologous proteins synthesized in Escherichia coli. Park JS, et al. Biotechnol Lett, 2007 Oct. PMID 17549433
  5. Direct transfer of NADH from malate dehydrogenase to complex I in Escherichia coli. Amarneh B, et al. Cell Biochem Biophys, 2005. PMID 15976458

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. GltA, icd and mdh do not form a transcription unit, are scattered around the chromosome and are expressed from different promoters.
    Title: Interaction of enzymes of the tricarboxylic acid cycle in Bacillus subtilis and Escherichia coli: a comparative study.
  2. The acetylation of lysine residues in MDH could enhance its enzyme activity.
    Title: Studying the Lysine Acetylation of Malate Dehydrogenase.
  3. The gene encoding malate dehydrogenase (MDH) was overexpressed in a pflB ldhA double mutant, NZN111, for succinic acid production.
    Title: Production of succinate by a pflB ldhA double mutant of Escherichia coli overexpressing malate dehydrogenase.
  4. N-terminus verified by Edman degradation on complete protein
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-malate dehydrogenase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables L-malate dehydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables malate dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables malate dehydrogenase activity IEA
Inferred from Electronic Annotation
more info
  
enables malate dehydrogenase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables oxidoreductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within anaerobic respiration IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in carboxylic acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within fermentation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within glycolytic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within malate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in malate metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tricarboxylic acid cycle IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tricarboxylic acid cycle IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extrinsic component of membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane HDA PubMed 

General protein information

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Preferred Names
malate dehydrogenase
NP_417703.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3383330..3384268 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417703.1 malate dehydrogenase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417703.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7LA59, A0A8S7YAY1
    Conserved Domains (1) summary
    PRK05086
    Location:1 → 312
    PRK05086; malate dehydrogenase; Provisional
Nucleotide Protein
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