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trpS tryptophan--tRNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947894, updated on 2-May-2024

Summary

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Gene symbol
trpS
Gene description
tryptophan--tRNA ligase
Primary source
ASAP:ABE-0011056
Locus tag
b3384
See related
ECOCYC:EG11030
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3371
Summary
Tryptophanyl-tRNA synthetase (TrpRS) is a member of the family of aminoacyl-tRNA synthetases, which interpret the genetic code by covalently linking amino acids to their specific tRNA molecules. [More information is available at EcoCyc: EG11030].
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Genomic context

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Sequence:
NC_000913.3 (3512634..3513638, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene PRD domain-containing protein YhfY Neighboring gene putative DNA-binding transcriptional regulator YhfZ Neighboring gene phosphoglycolate phosphatase Neighboring gene ribulose-phosphate 3-epimerase Neighboring gene DNA adenine methyltransferase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transfer RNA identity contributes to transition state stabilization during aminoacyl-tRNA synthesis. Ibba M, et al. Nucleic Acids Res, 1999 Sep 15. PMID 10471730, Free PMC Article
  2. Escherichia coli tryptophanyl-tRNA synthetase mutants selected for tryptophan auxotrophy implicate the dimer interface in optimizing amino acid binding. Sever S, et al. Biochemistry, 1996 Jan 9. PMID 8555191
  3. Role of the TIGN sequence in E. coli tryptophanyl-tRNA synthetase. Chan KW, et al. Biochim Biophys Acta, 1994 Apr 13. PMID 8155701
  4. Role of lysine-195 in the KMSKS sequence of E. coli tryptophanyl-tRNA synthetase. Chan KW, et al. FEBS Lett, 1995 Apr 17. PMID 7729548
  5. Evidence that uncharged tRNA can inhibit a programmed translational frameshift in Escherichia coli. Gao W, et al. J Mol Biol, 1995 Aug 11. PMID 7643397

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. An asymmetric structure of bacterial TrpRS supports the half-of-the-sites catalytic mechanism and facilitates antimicrobial screening.
    Title: An asymmetric structure of bacterial TrpRS supports the half-of-the-sites catalytic mechanism and facilitates antimicrobial screening.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Sequence homologies between the tryptophanyl tRNA synthetases of Bacillus stearothermophilus and Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables tryptophan-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tryptophan-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tryptophan-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in tRNA aminoacylation for protein translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
involved_in tryptophanyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tryptophanyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tryptophanyl-tRNA aminoacylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol EXP
Inferred from Experiment
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
tryptophan--tRNA ligase
NP_417843.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3512634..3513638 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417843.1 tryptophan--tRNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417843.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ACR5
    Conserved Domains (1) summary
    PRK00927
    Location:3 → 333
    PRK00927; tryptophanyl-tRNA synthetase; Reviewed
Nucleotide Protein
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Gene LinkOut

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