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mrcA peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947907, updated on 27-Nov-2023

Summary

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Gene symbol
mrcA
Gene description
peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA
Primary source
ASAP:ABE-0011082
Locus tag
b3396
See related
ECOCYC:EG10748
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3383; ponA
Summary
The N-terminal transglycosylase activity polymerizes the glycan backbone and is penicillin-insensitive. [More information is available at EcoGene: EG10748]. PBP1A, the product of the mrcA gene, is a bifunctional, inner membrane enzyme catalyzing the transglycosylation and transpeptidation of murein (peptidoglycan) precursors in the formation of the murein sacculus. [More information is available at EcoCyc: EG10748].
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Genomic context

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Sequence:
NC_000913.3 (3522871..3525423)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA utilization protein HofO Neighboring gene DNA utilization protein HofN Neighboring gene DNA utilization protein HofM Neighboring gene ADP-sugar diphosphatase NudE Neighboring gene inner membrane protein IgaA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Analysis of glycan polymers produced by peptidoglycan glycosyltransferases. Barrett D, et al. J Biol Chem, 2007 Nov 2. PMID 17704540, Free PMC Article
  2. Reforming L forms: they need part of a wall after all? Young KD. J Bacteriol, 2007 Sep. PMID 17644588, Free PMC Article
  3. Hybrid proteins of the transglycosylase and the transpeptidase domains of PBP1B and PBP3 of Escherichia coli. Zijderveld CA, et al. J Bacteriol, 1995 Nov. PMID 7592397, Free PMC Article
  4. Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A. Ishino F, et al. Biochem Biophys Res Commun, 1980 Nov 17. PMID 7006606
  5. Modulation of bacteriolysis by cooperative effects of penicillin-binding proteins 1a and 3 in Escherichia coli. Tuomanen E, et al. Antimicrob Agents Chemother, 1986 Nov. PMID 3541782, Free PMC Article

See all (57) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data indicate that outer-membrane-anchored lipoprotein LpoA has an elongated molecular shape, with dimensions sufficient to span from the outer membrane through the periplasm to interact with the peptidoglycan synthase PBP1A.
    Title: Elongated structure of the outer-membrane activator of peptidoglycan synthesis LpoA: implications for PBP1A stimulation.
  2. analysis of synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli
    Title: In vitro synthesis of cross-linked murein and its attachment to sacculi by PBP1A from Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables penicillin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables penicillin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables penicillin binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidoglycan glycosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables peptidoglycan glycosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidoglycan glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables serine-type carboxypeptidase activity RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in peptidoglycan biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in peptidoglycan biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of cell shape IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of cell shape NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in response to antibiotic IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within response to antibiotic IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3522871..3525423
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417855.4 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417855.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AND5, A0A827XTZ0, A0A8S7I2A8
    Conserved Domains (1) summary
    PRK11636
    Location:1 → 850
    mrcA; penicillin-binding protein 1a; Provisional
Nucleotide Protein
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