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zntA Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P-type ATPase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947972, updated on 4-Feb-2024

Summary

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Gene symbol
zntA
Gene description
Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P-type ATPase
Primary source
ECOCYC:EG12215
Locus tag
b3469
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3453; yhhO
Summary
HAD superfamily. [More information is available at EcoGene: EG12215]. The gene product of the yhhO gene, also referred to as zntA, is a P-type ATPase involved in the efflux of Pb(II), Cd(II), and Zn(II) . [More information is available at EcoCyc: EG12215].
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Genomic context

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Sequence:
NC_000913.3 (3606451..3608649)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2500 domain-containing protein YhhM Neighboring gene PF07947 family protein YhhN Neighboring gene sulfur transfer protein TusA Neighboring gene queuosine precursor transporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural Role of the First Four Transmembrane Helices in ZntA, a P(1B)-Type ATPase from Escherichia coli. Roberts CS, et al. Biochemistry, 2020 Dec 1. PMID 33190490
  2. Conservative and nonconservative mutations of the transmembrane CPC motif in ZntA: effect on metal selectivity and activity. Dutta SJ, et al. Biochemistry, 2007 Mar 27. PMID 17326661
  3. The metal-binding sites of the zinc-transporting P-type ATPase of Escherichia coli. Lys693 and Asp714 in the seventh and eighth transmembrane segments of ZntA contribute to the coupling of metal binding and ATPase activity. Okkeri J, et al. Biochim Biophys Acta, 2006 Nov. PMID 16890908
  4. Phospholipid requirement and pH optimum for the in vitro enzymatic activity of the E. coli P-type ATPase ZntA. Zimmer J, et al. Biochim Biophys Acta, 2006 May. PMID 16730648
  5. Conserved aspartic acid 714 in transmembrane segment 8 of the ZntA subgroup of P1B-type ATPases is a metal-binding residue. Dutta SJ, et al. Biochemistry, 2006 May 9. PMID 16669635

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural Role of the First Four Transmembrane Helices in ZntA, a P1B-Type ATPase from Escherichia coli.
    Title: Structural Role of the First Four Transmembrane Helices in ZntA, a P(1B)-Type ATPase from Escherichia coli.
  2. Data suggest that a kinetically-controlled transcription model is sufficient to explain the observed regulation of intracellular free zinc concentration by ZntR and ZntA after zinc shock.
    Title: ZntR-mediated transcription of zntA responds to nanomolar intracellular free zinc.
  3. Selectivity in ZntA possibly results from differences in metal-binding geometry that produce different structural responses.
    Title: Metal-binding affinity of the transmembrane site in ZntA: implications for metal selectivity.
  4. (693) and D(714) are functionally essential and appear to contribute to the metal specificity of ZntA, most probably by being parts of the metal-binding site made up by the CPC motif
    Title: The metal-binding sites of the zinc-transporting P-type ATPase of Escherichia coli. Lys693 and Asp714 in the seventh and eighth transmembrane segments of ZntA contribute to the coupling of metal binding and ATPase activity.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity ISA
Inferred from Sequence Alignment
more info
 PubMed 
enables ATPase-coupled monoatomic cation transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type cadmium transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables P-type cadmium transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type ion transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type zinc transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables P-type zinc transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables cadmium ion transmembrane transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lead ion transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cadmium ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within detoxification of zinc ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within intracellular zinc ion homeostasis IC
Inferred by Curator
more info
 PubMed 
acts_upstream_of_or_within lead ion transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in metal ion transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in monoatomic cation transport IEA
Inferred from Electronic Annotation
more info
  
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to cadmium ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to lead ion IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to zinc ion IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within zinc ion transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in zinc ion transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within zinc ion transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

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Preferred Names
Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P-type ATPase
NP_417926.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3606451..3608649
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417926.1 Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P-type ATPase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417926.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8T5YGE6
    Conserved Domains (1) summary
    NF033775
    Location:1 → 732
    P_type_ZntA; Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA
Nucleotide Protein
Heading Accession and Version

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