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glyS glycine--tRNA ligase subunit beta [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948080, updated on 2-May-2024

Summary

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Gene symbol
glyS
Gene description
glycine--tRNA ligase subunit beta
Primary source
ASAP:ABE-0011624
Locus tag
b3559
See related
ECOCYC:EG10410
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3547
Summary
glyS is an essential gene; the glyS mutants in the Keio collection have a second intact copy of glyS (R. [More information is available at EcoGene: EG10410]. A subunit cysteine thiol is not required for catalytic activity; however, C395 can be alkylated by NEM, resulting in loss of GlyRS activity . [More information is available at EcoCyc: EG10410].
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Genomic context

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Sequence:
NC_000913.3 (3722328..3724397, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene insertion element IS150 protein InsA Neighboring gene IS150 family conserved protein InsB Neighboring gene pseudo Neighboring gene glycine--tRNA ligase subunit alpha Neighboring gene putative lipoprotein YsaB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A sulfhydryl presumed essential is not required for catalysis by an aminoacyl-tRNA synthetase. Profy AT, et al. J Biol Chem, 1986 Nov 25. PMID 3536904
  2. Deletions in the large (beta) subunit of a hetero-oligomeric aminoacyl-tRNA synthetase. Toth MJ, et al. J Biol Chem, 1990 Jan 15. PMID 2404005
  3. Comparative X-ray structure analysis of human and Escherichia coli tRNA(Gly) acceptor stem microhelices. Förster C, et al. Biochem Biophys Res Commun, 2008 Apr 18. PMID 18275849
  4. Cell growth inhibition by sequence-specific RNA minihelices. Hipps D, et al. EMBO J, 1995 Aug 15. PMID 7664744, Free PMC Article
  5. Overproduction, purification, and subunit structure of Escherichia coli glycyl transfer ribonucleic acid synthetase. McDonald T, et al. Biochemistry, 1980 Apr 1. PMID 6992865

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. we present a comparison between the crystal structures of the eubacterial Escherichia coli and the human tRNA(Gly) acceptor stem microhelices and their surrounding hydration patterns.
    Title: Comparative X-ray structure analysis of human and Escherichia coli tRNA(Gly) acceptor stem microhelices.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aminoacyl-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables arginine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to glycine-tRNA ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycine-tRNA ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables tRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in arginyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in glycyl-tRNA aminoacylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycyl-tRNA aminoacylation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of glycine-tRNA ligase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of glycine-tRNA ligase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
glycine--tRNA ligase subunit beta
NP_418016.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3722328..3724397 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418016.1 glycine--tRNA ligase subunit beta [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418016.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827BT71
    Conserved Domains (1) summary
    TIGR00211
    Location:1 → 689
    glyS; glycyl-tRNA synthetase, tetrameric type, beta subunit
Nucleotide Protein
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Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
Research Materials