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waaF ADP-heptose--LPS heptosyltransferase 2 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948135, updated on 4-Apr-2024

Summary

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Gene symbol
waaF
Gene description
ADP-heptose--LPS heptosyltransferase 2
Primary source
ASAP:ABE-0011845
Locus tag
b3620
See related
ECOCYC:EG12210
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3610; rfaF
Summary
The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG12210].
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Genomic context

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Sequence:
NC_000913.3 (3794929..3795975)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein HtrL Neighboring gene ADP-L-glycero-D-mannoheptose 6-epimerase Neighboring gene ADP-heptose:LPS heptosyltransferase 1 Neighboring gene O-antigen ligase Neighboring gene protein YibX;protein YibX-S

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Kinetic Characterization and Computational Modeling of Escherichia coli Heptosyltransferase II: Exploring the Role of Protein Dynamics in Catalysis for GT-B Glycosyltransferase. Hassan BA, et al. Biochemistry, 2022 Aug 2. PMID 35861590
  2. Deletion of the heptosyltransferase genes rfaC and rfaF in Escherichia coli K-12 results in an Re-type lipopolysaccharide with a high degree of 2-aminoethanol phosphate substitution. Brabetz W, et al. Eur J Biochem, 1997 Jul 15. PMID 9266718
  3. Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis. Chen L, et al. J Bacteriol, 1993 May. PMID 8478319, Free PMC Article
  4. New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide. Coleman WG Jr, et al. J Bacteriol, 1985 Mar. PMID 3882666, Free PMC Article
  5. Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene. Pegues JC, et al. J Bacteriol, 1990 Aug. PMID 2198271, Free PMC Article

See all (24) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Kinetic Characterization and Computational Modeling of Escherichia coli Heptosyltransferase II: Exploring the Role of Protein Dynamics in Catalysis for GT-B Glycosyltransferase.
    Title: Kinetic Characterization and Computational Modeling of Escherichia coli Heptosyltransferase II: Exploring the Role of Protein Dynamics in Catalysis for GT-B Glycosyltransferase.
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Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ADP-heptose-lipopolysaccharide heptosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ADP-heptose-lipopolysaccharide heptosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ADP-heptose-lipopolysaccharide heptosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide core region biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lipopolysaccharide core region biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide core region biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ADP-heptose--LPS heptosyltransferase 2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3794929..3795975
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418077.1 ADP-heptose--LPS heptosyltransferase 2 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418077.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7EAG8
    Conserved Domains (1) summary
    PRK10916
    Location:1 → 348
    PRK10916; ADP-heptose--LPS heptosyltransferase RfaF
Nucleotide Protein
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Gene LinkOut

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