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asnA asparagine synthetase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948258, updated on 4-Apr-2024

Summary

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Gene symbol
asnA
Gene description
asparagine synthetase A
Primary source
ASAP:ABE-0012242
Locus tag
b3744
See related
ECOCYC:EG10091
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3738
Summary
AsnA and AsnB are redundant; an asparagine auxotrophy requires that both genes be mutated. [More information is available at EcoGene: EG10091]. Asparagine synthetase A (AsnA) is one of two asparagine synthetases in Escherichia coli, catalyzing the ammonia-dependent conversion of aspartate to asparagine. [More information is available at EcoCyc: EG10091].
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Genomic context

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Sequence:
NC_000913.3 (3927155..3928147)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene flavoprotein MioC Neighboring gene DNA-binding transcriptional dual regulator AsnC Neighboring gene protein ViaA Neighboring gene regulatory ATPase RavA Neighboring gene K(+):H(+) symporter Kup

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structure of asparagine synthetase reveals a close evolutionary relationship to class II aminoacyl-tRNA synthetase. Nakatsu T, et al. Nat Struct Biol, 1998 Jan. PMID 9437423
  2. The asparagine synthetase of Escherichia coli. II. Studies on mechanism. Cedar H, et al. J Biol Chem, 1969 Aug 10. PMID 4895362
  3. Overexpression and purification of asparagine synthetase from Escherichia coli. Sugiyama A, et al. Biosci Biotechnol Biochem, 1992 Mar. PMID 1369484
  4. Structural and functional properties of the Escherichia coli origin of DNA replication. Hirota Y, et al. Cold Spring Harb Symp Quant Biol, 1979. PMID 383377
  5. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. von Meyenburg K, et al. Cold Spring Harb Symp Quant Biol, 1979. PMID 383376

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables aspartate-ammonia ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables aspartate-ammonia ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables aspartate-ammonia ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in L-asparagine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in asparagine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in asparagine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within asparagine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
asparagine synthetase A
NP_418200.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3927155..3928147
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418200.1 asparagine synthetase A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418200.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7A6N7Q4, A0A826YCW9, A0A8S7EKH3, A0AA90FLT0
    Conserved Domains (1) summary
    COG2502
    Location:1 → 330
    AsnA; Asparagine synthetase A [Amino acid transport and metabolism]
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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