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rraA ribonuclease E inhibitor protein A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948419, updated on 4-Feb-2024

Summary

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Gene symbol
rraA
Gene description
ribonuclease E inhibitor protein A
Primary source
ASAP:ABE-0012837
Locus tag
b3929
See related
ECOCYC:EG11879
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3921; menG; yiiV
Summary
rraA (menG) was previously, erroneously, reported to encode demethylmenaquinone methyltransferase, in Genbank U56082. [More information is available at EcoGene: EG11879]. RraA inhibits ribonuclease E (RNase E, Rne) activity. [More information is available at EcoCyc: EG11879].
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Genomic context

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Sequence:
NC_000913.3 (4118845..4119330, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene glycerol facilitator Neighboring gene cell division factor ZapB Neighboring gene 1,4-dihydroxy-2-naphthoate octaprenyltransferase Neighboring gene ATPase component of the HslVU protease

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Roles of the 5'-phosphate sensor domain in RNase E. Garrey SM, et al. Mol Microbiol, 2011 Jun. PMID 21518390
  2. Effects of Escherichia coli RraA orthologs of Vibrio vulnificus on the ribonucleolytic activity of RNase E in vivo. Lee M, et al. Curr Microbiol, 2009 Apr. PMID 19194749
  3. Ribonuclease PH interacts with an acidic ribonuclease E site through a basic 80-amino acid domain. Martínez VP, et al. FEMS Microbiol Lett, 2014 Jun. PMID 24766456
  4. Potential regulatory interactions of Escherichia coli RraA protein with DEAD-box helicases. Pietras Z, et al. J Biol Chem, 2013 Nov 1. PMID 24045937, Free PMC Article
  5. The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome. Górna MW, et al. RNA, 2010 Mar. PMID 20106955, Free PMC Article

See all (27) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PNPase and RNase PH facilitate the stability and regulatory function of the sRNAs RyhB, CyaR, and MicA during exponential growth.
    Title: The Phosphorolytic Exoribonucleases Polynucleotide Phosphorylase and RNase PH Stabilize sRNAs and Facilitate Regulation of Their mRNA Targets.
  2. Authors narrowed down the sites of interaction in each enzyme to an acidic 16-amino acid region in the carboxy-terminal domain of RNase E and a basic 80-amino acid region in RNase PH including an alpha3 helix
    Title: Ribonuclease PH interacts with an acidic ribonuclease E site through a basic 80-amino acid domain.
  3. The phosphate sensor is required for efficient autoregulation of RNase E synthesis.
    Title: Roles of the 5'-phosphate sensor domain in RNase E.
  4. The truncated RNase E750 can still bind to Hfq although the truncation significantly reduces the Hfq-binding ability.
    Title: Hfq binding at RhlB-recognition region of RNase E is crucial for the rapid degradation of target mRNAs mediated by sRNAs in Escherichia coli.
  5. Data suggest that RraA can affect multiple components of the RNA degradosome in a dynamic, energy-dependent equilibrium.
    Title: The regulatory protein RraA modulates RNA-binding and helicase activities of the E. coli RNA degradosome.
  6. RraA and RraB have a conserved property to directly act on RNAse E-related enzymes and inhibit their ribonucleolytic activity.
    Title: Inhibitory effects of RraA and RraB on RNAse E-related enzymes imply conserved functions in the regulated enzymatic cleavage of RNA.
  7. expression of RraA or RraB is accompanied by specific changes in degradosome composition--and that these are in turn associated with alterations in RNA decay
    Title: Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome.
  8. Findings suggest RraA is able to effectively modulate the catalytic activity of RNase E in vivo.
    Title: RraA rescues Escherichia coli cells over-producing RNase E from growth arrest by modulating the ribonucleolytic activity.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables endoribonuclease inhibitor activity IEA
Inferred from Electronic Annotation
more info
  
enables enzyme binding IEA
Inferred from Electronic Annotation
more info
  
enables enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribonuclease inhibitor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribonuclease inhibitor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in negative regulation of RNA catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within negative regulation of RNA catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein homotrimerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of RNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-containing complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ribonuclease E inhibitor protein A

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4118845..4119330 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418364.1 ribonuclease E inhibitor protein A [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418364.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AR25
    Conserved Domains (1) summary
    PRK09372
    Location:1 → 159
    PRK09372; ribonuclease E inhibitor RraA
Nucleotide Protein
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