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mdtM multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948861, updated on 11-Apr-2024

Summary

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Gene symbol
mdtM
Gene description
multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Primary source
ASAP:ABE-0014221
Locus tag
b4337
See related
ECOCYC:EG12576
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4328; yjiO
Summary
Major Facilitator Superfamily (MFS). [More information is available at EcoGene: EG12576]. The YjiO protein is, based on sequence similarity and hydropathy analysis, a member of the major facilitator superfamily (MFS) . [More information is available at EcoCyc: EG12576].
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Genomic context

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Sequence:
NC_000913.3 (4567287..4568519, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative dehydratase subunit Neighboring gene DUF445 domain-containing protein YjiN Neighboring gene protein YtiE Neighboring gene pseudo

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A single-component multidrug transporter of the major facilitator superfamily is part of a network that protects Escherichia coli from bile salt stress. Paul S, et al. Mol Microbiol, 2014 May. PMID 24684269, Free PMC Article
  2. Multidrug resistance protein MdtM adds to the repertoire of antiporters involved in alkaline pH homeostasis in Escherichia coli. Holdsworth SR, et al. BMC Microbiol, 2013 May 23. PMID 23701827, Free PMC Article
  3. Functional and biochemical characterisation of the Escherichia coli major facilitator superfamily multidrug transporter MdtM. Holdsworth SR, et al. Biochimie, 2012 Jun. PMID 22426385
  4. MdfA, an Escherichia coli multidrug resistance protein with an extraordinarily broad spectrum of drug recognition. Edgar R, et al. J Bacteriol, 1997 Apr. PMID 9079913, Free PMC Article
  5. Influence of the yjiL-mdtM Gene Cluster on the Antibacterial Activity of Proline-Rich Antimicrobial Peptides Overcoming Escherichia coli Resistance Induced by the Missing SbmA Transporter System. Krizsan A, et al. Antimicrob Agents Chemother, 2015 Oct. PMID 26169420, Free PMC Article

See all (21) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MdtM, a 410 amino acid residue protein that belongs to the large and ubiquitous major facilitator superfamily. [review]
    Title: Clamping down on drugs: the Escherichia coli multidrug efflux protein MdtM.
  2. The yjiL-mdtM gene cluster mediates the antibacterial activity of proline-rich antimicrobial peptides overcoming Escherichia coli resistance induced by the missing SbmA transporter system.
    Title: Influence of the yjiL-mdtM Gene Cluster on the Antibacterial Activity of Proline-Rich Antimicrobial Peptides Overcoming Escherichia coli Resistance Induced by the Missing SbmA Transporter System.
  3. MdtM, a single-component multidrug resistance transporter of the major facilitator superfamily, functions in bile salt resistance in Escherichia coli by catalysing secondary active transport of bile salts out of the cell cytoplasm.
    Title: A single-component multidrug transporter of the major facilitator superfamily is part of a network that protects Escherichia coli from bile salt stress.
  4. Taken together, the results support a role for MdtM in alkaline pH tolerance. MdtM can therefore be added to the currently limited list of antiporters known to function in pH homeostasis in the model organism E. coli.
    Title: Multidrug resistance protein MdtM adds to the repertoire of antiporters involved in alkaline pH homeostasis in Escherichia coli.
  5. Purified MdtM is monomeric and stable in dodecyl-beta-D-maltopyranoside solution and binds chloramphenicol with nanomolar affinity in the same detergent.
    Title: Functional and biochemical characterisation of the Escherichia coli major facilitator superfamily multidrug transporter MdtM.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables bile acid transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables bile acid transmembrane transporter activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables potassium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within bile acid and bile salt transport IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within bile acid and bile salt transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within potassium ion export across plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in potassium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proton transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of cellular pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to antibiotic IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within sodium ion export across plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in xenobiotic detoxification by transmembrane export across the plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within xenobiotic detoxification by transmembrane export across the plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 

General protein information

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Preferred Names
multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4567287..4568519 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418757.1 multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418757.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A376X7E5
    Conserved Domains (1) summary
    PRK15403
    Location:1 → 410
    PRK15403; multidrug efflux MFS transporter MdtM
Nucleotide Protein
Heading Accession and Version

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Other Literature Sources
Molecular Biology Databases
Research Materials