PXD013116

PXD013116 is an original dataset announced via ProteomeXchange.

Title	Asparagine hydroxylation is likely to be a reversible post-translational modification
Description	Amino acid hydroxylation is a common post-translational modification, which generally regulates protein interactions or adds a functional group that can be further modified. Such hydroxylation is currently considered irreversible, necessitating the degradation and re-synthesis of the entire protein to reset the modification. Here we present evidence that the cellular machinery can reverse FIH-mediated asparagine hydroxylation on intact proteins. These data suggest that asparagine hydroxylation is a flexible and dynamic post-translational modification akin to modifications involved in regulating signalling networks, such as phosphorylation, methylation and ubiquitylation.
HostingRepository	PRIDE
AnnounceDate	2020-03-23
AnnouncementXML	Submission_2020-08-09_23:06:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-03-18 04:19:07	ID requested
1	2020-03-23 00:40:38	announced
⏵ 2	2020-08-09 23:06:22	announced	2020-08-10: Updated publication reference for PubMed record(s): 32759169.

Rodriguez J, Haydinger CD, Peet DJ, Nguyen LK, von Kriegsheim A, Asparagine Hydroxylation is a Reversible Post-translational Modification. Mol Cell Proteomics, 19(11):1777-1789(2020) [pubmed]

submitter keyword: LC-MS/MS, hydroxylation, hypoxia, PTM

Alex von Kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@igmm.ed.ac.uk
lab head
Alex von kriegsheim
contact affiliation	Systems Biology
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD013116

PRIDE project URI

• PRIDE
  1. PXD013116
     1. Label: PRIDE project
     2. Name: Asparagine hydroxylation is likely to be a reversible post-translational modification