Rhea - reaction knowledgebase

Enzymes

UniProtKB ^help_outline	12,561 proteins
Enzyme classes ^help_outline	EC 1.1.1.38 malate dehydrogenase (oxaloacetate-decarboxylating) EC 1.1.1.40 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) EC 4.1.1.112 oxaloacetate decarboxylase
GO Molecular Function ^help_outline	GO:0008948 oxaloacetate decarboxylase activity

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Name ^help_outline oxaloacetate Identifier CHEBI:16452 (CAS: 149-63-3) ^help_outline Charge -2 Formula C4H2O5 InChIKey^help_outline KHPXUQMNIQBQEV-UHFFFAOYSA-L SMILES^help_outline [O-]C(=O)CC(=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 61 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline H⁺ Identifier CHEBI:15378 Charge 1 Formula H InChIKey^help_outline GPRLSGONYQIRFK-UHFFFAOYSA-N SMILES^help_outline [H+] 2D coordinates Mol file for the small molecule Search links Involved in 9,717 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline pyruvate Identifier CHEBI:15361 (CAS: 57-60-3) ^help_outline Charge -1 Formula C3H3O3 InChIKey^help_outline LCTONWCANYUPML-UHFFFAOYSA-M SMILES^help_outline CC(=O)C([O-])=O 2D coordinates Mol file for the small molecule Search links Involved in 219 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule
Name ^help_outline CO₂ Identifier CHEBI:16526 (CAS: 124-38-9) ^help_outline Charge 0 Formula CO2 InChIKey^help_outline CURLTUGMZLYLDI-UHFFFAOYSA-N SMILES^help_outline O=C=O 2D coordinates Mol file for the small molecule Search links Involved in 1,032 reaction(s) Find molecules that contain or resemble this structure Find proteins in UniProtKB for this molecule

Cross-references

	RHEA:15641	RHEA:15642	RHEA:15643	RHEA:15644
Reaction direction	undefined	left-to-right	right-to-left	bidirectional
UniProtKB ^help_outline	12,561 proteins	6 proteins
EC numbers ^help_outline	1.1.1.38 1.1.1.40 4.1.1.112
Gene Ontology ^help_outline	GO:0008948
KEGG ^help_outline				R00217
MetaCyc ^help_outline		OXALODECARB-RXN
EcoCyc ^help_outline		OXALODECARB-RXN
Reactome ^help_outline		R-HSA-9012016.4 R-HSA-9861660.2

Publications

Purification and properties of pigeon liver malic enzyme.

RUTTER W.J., LARDY H.A.

J Biol Chem 233:374-382(1958) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Biosynthesis of dicarboxylic acids by carbon dioxide fixation. V. Further study of the "malic" enzyme of Lactobacillus arabinosus.

KAUFMAN S., KORKES S., DEL CAMPILLO A.

J Biol Chem 192:301-312(1951) [PubMed] [EuropePMC]
Physiological studies on acid metabolism. 7. Malic enzyme from Kalanchoe crenata: effects of carbon dioxide concentration.

WALKER D.A.

Biochem J 74:216-223(1960) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Some properties of the malic enzyme of pigeon liver. 2. Synthesis of malate from pyruvate.

STICKLAND R.G.

Biochem J 73:654-659(1959) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Some properties of the malic enzyme of pigeon liver. 1. Conversion of malate into pyruvate.

STICKLAND R.G.

Biochem J 73:646-654(1959) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Biosynthesis of dicarboxylic acids by carbon dioxide fixation; isolation and properties of an enzyme from pigeon liver catalyzing the reversible oxidative decarboxylation of 1-malic acid.

OCHOA S., MEHLER A.H., KORNBERG A.

J Biol Chem 174:979-1000(1948) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Biosynthesis of dicarboxylic acids by carbon dioxide fixation. VII. Equilibrium of malic enzyme reaction.

HARARY I., KOREY S.R., OCHOA S.

J Biol Chem 203:595-604(1953) [PubMed] [EuropePMC]

This publication is cited by 1 other entry.
Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli.

Yamaguchi M.

Reactions catalyzed by NAD-linked malic enzyme from Escherichia coli were investigated. In addition to L-malate oxidative decarboxylase activity (Activity 1) and oxaloacetate decarboxylase activity (Activity 2), the enzyme exhibited oxaloacetate reductase activity (Activity 3) and pyruvate reducta ... >> More

Reactions catalyzed by NAD-linked malic enzyme from Escherichia coli were investigated. In addition to L-malate oxidative decarboxylase activity (Activity 1) and oxaloacetate decarboxylase activity (Activity 2), the enzyme exhibited oxaloacetate reductase activity (Activity 3) and pyruvate reductase activity (Activity 4). Optimum pH's for Activities 3 and 4 were 4.0 and 5.0, and their specific activities were 1.7 and 0.07, respectively. Upon reaction with N-ethylmaleimide (NEM), Activity 1 decreased following pseudo-first order kinetics. Activity 2 decreased in parallel with Activity 1, while Activities 3 and 4 were about ten-fold enhanced by NEM modification. Modification of one or two sulfhydryl groups per enzyme subunit caused an alteration of the activities. Tartronate, a substrate analog, NAD+, and Mn2+ protected the enzyme against the modification. The Km values for the substrates and coenzymes were not significantly affected by NEM modification. Similarly, other sulfhydryl reagents such as p-hydroxymercuribenzoate (PMB), 5,5'-dithiobis(2-nitrobenzoate) (DTNB), and iodoacetate inhibited the decarboxylase activities and activated the reductase activities to various extents. Modification of the enzyme with PMB or DTNB was reversed by the addition of a sulfhydryl compound such as dithiothreitol or 2-mercaptoethanol. Based on the above results, the mechanism of the alteration of enzyme activities by sulfhydryl group modification is discussed. << Less

J Biochem 86:325-333(1979) [PubMed] [EuropePMC]

RHEA:15641 oxaloacetate + H(+) = pyruvate + CO2

Enzymes

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Cross-references

Publications

Purification and properties of pigeon liver malic enzyme.

Biosynthesis of dicarboxylic acids by carbon dioxide fixation. V. Further study of the "malic" enzyme of Lactobacillus arabinosus.

Physiological studies on acid metabolism. 7. Malic enzyme from Kalanchoe crenata: effects of carbon dioxide concentration.

Some properties of the malic enzyme of pigeon liver. 2. Synthesis of malate from pyruvate.

Some properties of the malic enzyme of pigeon liver. 1. Conversion of malate into pyruvate.

Biosynthesis of dicarboxylic acids by carbon dioxide fixation; isolation and properties of an enzyme from pigeon liver catalyzing the reversible oxidative decarboxylation of 1-malic acid.

Biosynthesis of dicarboxylic acids by carbon dioxide fixation. VII. Equilibrium of malic enzyme reaction.

Studies on regulatory functions of malic enzymes. IV. Effects of sulfhydryl group modification on the catalytic function of NAD-linked malic enzyme from Escherichia coli.