Publications

• The novel transmembrane Escherichia coli proteins involved in the amino acid efflux.

  Zakataeva N.P., Aleshin V.V., Tokmakova I.L., Troshin P.V., Livshits V.A.

  A novel gene of Escherichia coli, rhtB, has been characterized. Amplification of this gene provides resistance to homoserine and homoserine lactone. Another E. coli gene, rhtC, provides resistance to threonine. The homologues of RhtB are widely distributed among various eubacteria and archaea, fro ... >> More

  A novel gene of Escherichia coli, rhtB, has been characterized. Amplification of this gene provides resistance to homoserine and homoserine lactone. Another E. coli gene, rhtC, provides resistance to threonine. The homologues of RhtB are widely distributed among various eubacteria and archaea, from one to 12 copies of family members that differ in their primary structure were found in the genomes. Most of them are genes that encode hypothetical transmembrane proteins. Experimental data that indicate participation of the rhtB product in the excretion of homoserine have been obtained. << Less

  FEBS Lett. 452:228-232(1999) [PubMed] [EuropePMC]

  This publication is cited by 2 other entries.

• Influence of threonine exporters on threonine production in Escherichia coli.

  Kruse D., Kramer R., Eggeling L., Rieping M., Pfefferle W., Tchieu J.H., Chung Y.J., Jr Saier M.H., Burkovski A.

  Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine- ... >> More

  Threonine production in Escherichia coli threonine producer strains is enhanced by overexpression of the E. coli rhtB and rhtC genes or by heterologous overexpression of the gene encoding the Corynebacterium glutamicum threonine excretion carrier, thrE. Both E. coli genes give rise to a threonine-resistant phenotype when overexpressed, and they decrease the accumulation of radioactive metabolites derived from [(14)C] L-threonine. The evidence presented supports the conclusion that both RhtB and RhtC catalyze efflux of L-threonine and other structurally related neutral amino acids, but that the specificities of these two carriers differ substantially. << Less

  Appl Microbiol Biotechnol 59:205-210(2002) [PubMed] [EuropePMC]

• Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli.

  Livshits V.A., Zakataeva N.P., Aleshin V.V., Vitushkina M.V.

  The rhtA gene known as the ybiF ORF in the genome of Escherichia coli was identified as a new gene involved in threonine and homoserine efflux. This gene encodes a highly hydrophobic membrane protein that contains 10 predicted transmembrane segments. The rhtA23 mutation, which is an A-for-G substi ... >> More

  The rhtA gene known as the ybiF ORF in the genome of Escherichia coli was identified as a new gene involved in threonine and homoserine efflux. This gene encodes a highly hydrophobic membrane protein that contains 10 predicted transmembrane segments. The rhtA23 mutation, which is an A-for-G substitution at position -1 in relation to the ATG start codon, increases the expression level of the rhtA gene. The overexpression of rhtA gene results in resistance to inhibitory concentrations of homoserine, threonine and a variety of other amino acids and amino acid analogues, reduced threonine and homoserine accumulation in resistant cells and increased production of threonine, homoserine, lysine and proline by the respective producing strains. The RhtA protein belongs to a vast family of transporters. The genome of E. coli contains at least 10 paralogues of RhtA. Phylogenetic analysis indicates that a common ancestor of living organisms contained several RhtA homologues. << Less

  Res. Microbiol. 154:123-135(2003) [PubMed] [EuropePMC]

  This publication is cited by 1 other entry.