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ligA DNA ligase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946885, updated on 4-Apr-2024

Summary

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Gene symbol
ligA
Gene description
DNA ligase
Primary source
ASAP:ABE-0007938
Locus tag
b2411
See related
ECOCYC:EG10534
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dnaL; ECK2406; lig; lop; pdeC
Summary
ligA is an essential gene. [More information is available at EcoGene: EG10534]. LigA is one of two known NAD(+)-dependent DNA ligases, catalyzing the formation of phosphodiester bonds in duplex DNA. [More information is available at EcoCyc: EG10534].
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Genomic context

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Sequence:
NC_000913.3 (2528161..2530176, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative DNA-binding transcriptional regulator YfeR Neighboring gene putative solute:Na(+) symporter Neighboring gene PF12843 family protein YpeB Neighboring gene cell division protein ZipA Neighboring gene sulfate:H(+) symporter

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA). Alomari A, et al. Molecules, 2021 Apr 25. PMID 33923034, Free PMC Article
  2. Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA). Chauleau M, et al. Nucleic Acids Res, 2016 Mar 18. PMID 26857547, Free PMC Article
  3. Chromosome demise in the wake of ligase-deficient replication. Kouzminova EA, et al. Mol Microbiol, 2012 Jun. PMID 22582878, Free PMC Article
  4. Structure-guided Mutational Analysis of the Nucleotidyltransferase Domain of Escherichia coli DNA Ligase (LigA). Wang LK, et al. J Biol Chem, 2009 Mar 27. PMID 19150981, Free PMC Article
  5. Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase. Wang LK, et al. J Biol Chem, 2008 Aug 22. PMID 18515356, Free PMC Article

See all (84) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA).
    Title: Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA).
  2. Ensemble and single-molecule experiments reveal that phage Mu Gam protein binds to DNA ends, slows the progress of RecBCD exonuclease, promotes binding of NAD(+)-dependent ligase A, and stimulates ligation.
    Title: Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligase.
  3. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described.
    Title: Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA).
  4. Here the authors show that replication without ligase kills after a short resistance period.
    Title: Chromosome demise in the wake of ligase-deficient replication.
  5. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation
    Title: Structure-guided mutational analysis of the OB, HhH, and BRCT domains of Escherichia coli DNA ligase.
  6. N-terminus verified by Edman degradation on complete protein
    Title: Nucleotide sequence of the lig gene and primary structure of DNA ligase of Escherichia coli.
  7. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding.
    Title: Analysis of ligation and DNA binding by Escherichia coli DNA ligase (LigA).
  8. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition
    Title: Last stop on the road to repair: structure of E. coli DNA ligase bound to nicked DNA-adenylate.
  9. temperature sensitive mutant characterized
    Title: Characterization of a temperature-sensitive DNA ligase from Escherichia coli.
  10. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA)
    Title: Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA).
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA ligase (NAD+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA ligase (NAD+) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA ligase (NAD+) activity IEA
Inferred from Electronic Annotation
more info
  
enables NAD+ binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA ligation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in base-excision repair, DNA ligation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within base-excision repair, DNA ligation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
DNA ligase
NP_416906.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2528161..2530176 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416906.1 DNA ligase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416906.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A827XU11, F4V4E8
    Conserved Domains (1) summary
    PRK07956
    Location:1 → 670
    ligA; NAD-dependent DNA ligase LigA; Validated
Nucleotide Protein
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Gene LinkOut

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